Table 2.
SHIRPA scores. At 1 mpi, mice performed SHIRPA. SHIRPA responses were scored on a scale of 0–3 (0 = no response, 1 = slow response; 2 = normal response; 3 = hyper response). Ratios show the number of mice with normal (Score of 2) versus abnormal scores (Score of 0, 1, or 3) (normal/abnormal) between each group and for each individual SHIRPA response. Abnormal percentages were calculated for each group and for each SHIRPA response tested. Shaded boxes are SHIRPA responses with 50% or greater abnormal responses.
| SHIRPA Response | Body position | Spontaneous activity | Respiratory rate | Touch escape | Transfer arousal | Gait | Piloerection | Visual placing | Trunk curl | Fear |
|---|---|---|---|---|---|---|---|---|---|---|
| WT Saline | 8/0 | 6/2 | 8/0 | 6/2 | 6/2 | 8/0 | 8/0 | 8/0 | 5/3 | 8/0 |
| Tg2576 Saline | 8/0 | 5/3 | 7/1 | 5/3 | 5/3 | 8/0 | 8/0 | 8/0 | 5/3 | 6/2 |
| WT VEEV TC-83 | 7/2 | 4/5 | 9/0 | 4/5 | 4/5 | 5/4 | 6/3 | 7/2 | 5/4 | 7/2 |
| Tg2576 VEEV TC-83 | 3/5 | 3/5 | 2/6 | 0/8 | 0/8 | 4/4 | 6/2 | 3/5 | 3/5 | 0/8 |
| Abnormal Percentage | ||||||||||
| WT Saline | 0% | 25% | 0% | 25% | 25% | 0% | 0% | 0% | 37.5% | 0% |
| Tg2576 Saline | 0% | 37.5% | 12.5% | 37.5% | 37.5% | 0% | 0% | 0% | 37.5% | 25% |
| WT VEEV TC-83 | 22.2% | 55.5% | 0% | 55.5% | 55.5% | 44.4% | 33% | 22.2% | 44.4% | 22.2% |
| Tg2576 VEEV TC-83 | 62.5% | 62.5% | 75% | 100% | 100% | 50% | 25% | 62.5% | 62.5% | 100% |