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. 2024 May 3;24:557. doi: 10.1186/s12885-024-12313-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — FGL1 knockdown inhibits invasion and migration of ESCC cells in vitro and in vivo. (A-C) FGL1 expression levels were assessed in ECA109 cells transfected with FGL1 shRNA (FGL1-KD) or random nonsense sequences (NC) using qRT-PCR (A) and western blotting (B and C). β-Actin served as the loading control. (D) The effects of FGL1 knockdown on ESCC cells invasion were evaluated by transwell invasion assay. Scale bars, 100 μm. (E) The effects of FGL1 knockdown on ESCC cells migration were evaluated by wound healing assay. Scale bars, 100 μm. (F and G) FGL1 knockdown resulted in decreased lung metastasis, shown by the representative image (F) and lung nodules number (G) of lung metastasis in nude mice bearing ECA109 cells. The statistical difference was assessed with one-way ANOVA followed by Dunnett tests in A, C, D, E and G. Error bars show the SD from three independent experiments. **p < 0.01, ****p < 0.0001