TABLE 2.
Rat Se-GPx1 RNA in transiently transfected mouse NIH 3T3 cells
| Codon 46 | Se | %a of:
|
||||
|---|---|---|---|---|---|---|
| premRNAN | mRNAN | mRNAC | mRNAN/ pre-mRNAN | mRNAC/ mRNAN | ||
| Sec (UGA) | − | 73 | 237 | 52 | 105 | 50 |
| + | 84 | 260 | 100 | 100 | 100 | |
| Ter (UAA) | − | 39 | 138 | 34 | 114 | 30 |
| + | 50 | 194 | 30 | 102 | 29 | |
| Cys (UGC) | − | 145 | 502 | 157 | 110 | 143 |
| + | 157 | 487 | 154 | 101 | 153 | |
a
Values derive from a quantitative analysis of the experimental results shown in Fig. 4. The levels of nuclear pre-mRNA (pre-mRNAN), nuclear mRNA (mRNAN), and cytoplasmic mRNA (mRNAC) were normalized to the corresponding level of β-globin mRNA and calculated as percentages of the normalized value for the cytoplasmic mRNA Sec (UGA) in Se-supplemented (+) medium, which was given a value of 100%. Each ratio (mRNAN/pre-mRNAN and mRNAC/mRNAN) was calculated as a percentage of the corresponding ratio for mCMV-GPx1-UGA(46)RNA in Se-supplemented (+) medium, which was given a value of 100.