Table 3.
Comparison of metabolites between the fluoride-resistant strain and the wild-type strain in stationary phase
| Metabolite | Chemical formula | Rt RP (s)† | Rt HILIC (s)‡ | m/z | VIP§ | Trend¶ | Metabolic pathway | HMDB ID | KEGG ID |
|---|---|---|---|---|---|---|---|---|---|
| 1-octadecanoyl-sn-glycerol 3-phosphate | C21H41O7P1 | 613.6 | - | 437 | 2.21 | ↑ | Fatty acid metabolism | HMDB07854 | - |
| 2,3-diphosphoglycerate | C3H3O10P2 | - | 206.7 | 261 | 2.66 | ↑ | Carbohydrate metabolism | HMDB01294 | C01159 |
| 3-keto-L-gulonate 6-phosphate | C6H8O10P1 | 1041.7 | - | 270 | 2.29 | ↓ | Carbohydrate metabolism | - | C14899 |
| 6-phospho-D-glucono-1,5-lactone | C6H9O9P1 | - | 110.6 | 256 | 3.16 | ↑ | Carbohydrate metabolism | HMDB01127 | C01236 |
| Aminopropyl cadaverine | C8H24N3 | 120.0 | 508.8 | 162 | 2.87 | ↓ | Amino acid metabolism | HMDB12189 | C16565 |
| CMP | C9H12N3O8P1 | 1411.1 | - | 321 | 2.82 | ↓ | Nucleotide metabolism | HMDB00095 | C00055 |
| dCMP | C9H12N3O7P1 | - | 207.8 | 305 | 2.19 | ↑ | Nucleotide metabolism | HMDB01202 | C00239 |
| D-ribose-5-phosphate | C5H9O8P1 | 794.0 | - | 228 | 2.98 | ↓ | Carbohydrate metabolism | HMDB01548 | C00117 |
| D-sedoheptulose-1,7-bisphosphate | C7H12O13P2 | 1677.6 | - | 366 | 3.26 | ↓ | Carbohydrate metabolism | HMDB60274 | - |
| dUDP | C9H11N2O11P2 | - | 400.9 | 385 | 2.15 | ↑ | Nucleotide metabolism | HMDB01000 | C01346 |
| Fructose-1,6-bisphosphate | C6H10O12P2 | 1221.4 | - | 336 | 2.61 | ↑ | Carbohydrate metabolism | HMDB01058 | - |
| GMP | C10H12N5O8P1 | 1410.3 | - | 360 | 2.67 | ↓ | Nucleotide metabolism | HMDB01397 | C00144 |
| Pyridoxamine 5’-phosphate | C8H12N2O5P1 | 1042.5 | 114.6 | 247 | 2.03 | ↑ | Amino acid metabolism | HMDB01555 | C00647 |
†Rt RP (s), retention time of reversed phase column (second)
‡Rt HILIC (s), retention time of hydrophilic interaction liquid chromatography column (second)
§VIP, variable important on projection; VIP was obtained from PLS-DA with a threshold of 2.0
¶Trend: The levels of the discriminant metabolites were labeled with (↓) downregulated and (↑) upregulated when the fluoride-resistant strain compared to the wild-type strain (Mann–Whitney test, p < 0.05)