Figure 4.

Domain mapping of TRIM56 determinants required for its augmentation of TLR3 signaling to activation of NF-κB–dependent promoter. HEK293-TLR3 cells were transfected with an empty vector (“vector”) or the indicated plasmid encoding WT or various mutant TRIM56-V5, along with internal control plasmid pRL-TK and NF-κB responsive reporter plasmid PRDII-Luc, followed by mock- or poly-I:C-stimulation for 8 h and dual luciferase reporter assay. Statistical analysis was performed between WT TRIM56 and vector or mutant TRIM56 after poly-I:C stimulation. Double asterisks denote that statistical differences exist with a p value of < 0.01. N.S., not statistically significant; pIC, poly-I:C. TLR, Toll-like receptor; TRIM56, tripartite-motif protein-56.