. 2024 Mar 29;300(5):107249. doi: 10.1016/j.jbc.2024.107249

Table 1.

Primers for PCR mutagenesis of human (hT56) and murine TRIM56 (mT56)

Mutation(s)	Primer sequences (5′->3′)
hT56-Ser⁴⁶⁹Ala	Forward: ATATCCCGGGAGCCCAGCCCAG Reverse: CGCCTTGAGCCTGCCTTTGAAC
hT56-Ser⁴⁷¹Ala	Forward: GCTCGAGAGCCCAGCCCAGCCCT Reverse: AATTGACTTGAGCCTGCCTTTGA
hT56-Ser⁴⁷⁵Ala	Forward: GCTCCAGCGCTGGGGCCGAATCT Reverse: GGGCTCCCGGGAAATTGACTTGA
hT56-Ser⁷¹⁰Ala	Forward: GCTCTCCTTGGAGACTTCCTG Reverse: TCCCTTCGGGTCCAGGATCAC
hT56-Ser⁷¹⁰Asp	Forward: GATCTCCTTGGAGACTTCCTG Reverse: TCCCTTCGGGTCCAGGATCAC
hT56-Ser⁴⁶⁹Ala+Ser⁴⁷¹Ala+Ser⁴⁷⁵Ala	Forward: GAGCCCGCTCCAGCCCTGGGGCCGAAT Reverse: TCGAGCAATAGCCTTGAGCCTGCCTTTGA
mT56-Ser⁶⁸⁹Ala	Forward: GCTCTTCTTGGTGACTTCCTAAC Reverse: TCCCTTGGGATCCAGTATCAC