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. 2024 Apr 2;300(5):107257. doi: 10.1016/j.jbc.2024.107257

Figure 1.

Figure 1

RAP2 mediates Hippo regulation by environmental signals and stresses.A, Wild-type (WT) and RAP2A/B/C triple knockout (tKO) cells were treated with cAMP inducers IBMX and FSK for 30 and 60 min. Then the cell lysates were collected for analyses of LATS phosphorylation at hydrophobic motif (HM) and YAP phosphorylation at Serine 127. The results of the Western blot analysis are quantified and displayed beneath each corresponding band. B, WT and RAP2A/B/C tKO cells were serum starved, by replacing 10% FBS DMEM with serum-free DMEM (-FBS), for 30 and 60 min. C, WT and RAP2A/B/C tKO cells were treated with the Rho inhibitor C3 for 30 and 60 min for analyses of the role of RAP2 in Hippo regulation by Rho GTPase. D, WT and RAP2A/B/C tKO cells were treated with an energy stress-inducing agent 2-deoxy-D-glucose (2-DG) for 30 and 60 min. E, WT and RAP2A/B/C tKO cells were treated with an energy stress-inducing agent sorbitol for 30 and 60 min. F, a diagram of a working model illustrating the role of RAP2 GTPase in the Hippo signaling regulation by environmental signals and stress.