RAP2 is a required component of the MAP4K-mediated non-canonical Hippo signaling (Hpo2) to respond to environmental signals.A, a diagram illustrating the role of RAP2 in the canonical MST1/2-mediated (Hpo1) and the non-canonical MAP4K-mediated (Hpo2) signaling pathways. B, WT, RAP2A/B/C tKO, MST1/2 dKO, and RAP2A/B/C-MST1/2 5KO cells were treated with low dose (12.5 mM) and high dose (25.0 mM) of 2-DG. C, WT, RAP2A/B/C tKO, MST1/2 dKO, and RAP2A/B/C-MST1/2 5KO cells were serum-starved for 30 and 60 min.