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. 2024 Apr 2;300(5):107257. doi: 10.1016/j.jbc.2024.107257

Figure 7.

Figure 7

RAP2 GTPase is involved in Hippo signaling regulation by tubulin cytoskeleton dynamics.A, WT, RAP2A/B/C tKO, and MAP4K4/6/7 tKO HEK293A cells were treated with Taxol (3 μM) for 120 min. B and C, Representative bright filed images (B) and cell viability MTS test (C) showed cell survival of WT and RAP2A/B/C tKO MCF10AT cells treated with 50 nM of Taxol for 24 h. For the MTS assay, a Two-way ANOVA and Tukey's multiple comparison test was applied. Scale bar, 300 μm. The data are reported as mean ± SD. D, transwell migration assay on WT and RAP2A/B/C tKO MCF10AT cells treated with 50 nM of Taxol for 24 h. Scale bar, 100 μm. E, quantification of cell migration assay. The data are reported as mean ± SD. Two-way ANOVA and Tukey's multiple comparison tests were used.