FIG. 1.

The E box in the bp −126 and −86 region is a specific E1A target. Schematic representations of the insulin:LUC constructs with the C1 (−115 to −107 bp), RIPE3a2 (−108 to −99 bp), and E (−100 to −91 bp) factor binding sites (64). HIT T-15 cells were transfected with 2.5 μg of insulin:LUC and 2.5 μg of either E1A or the CMV4 vector. The activity ± standard error of the mean from three to five independent experiments is presented relative to the insulin:LUC construct alone. The activity of each of the insulin-driven constructs was similar to that previously reported (58, 63); values for RIPE3, RIPE3 Em, E, C1, and E box were 150,473, 45,667, 9,753, 8,942, and 4,000 relative light units, respectively.