Figure 6.

Identification of additional FACS-style markers for homogeneous B cell sorting. (A) Strategy for additional marker selection for naïve, unswitched memory, and switched memory B cells, performed on B cells from all disease states and healthy individuals combined. (B) Additional gates for (top) naïve B cells, (middle) unswitched memory B cells and (bottom) switched memory B cells. (C) Additional gates for IgD- CD27- B cells. (D) Heatmap of the heterogeneity of B cells captured using the additional gates. The number represents the number of cells captured with in the corresponding flow-cytometric gate with the corresponding multi-omics label. (E) Table of the accuracy, sensitivity, specificity, purity and percentage increase in purity of the flow cytometric-style gating to capture target B cell populations using the additional gates compared to the corresponding multi-omics labels. (F) Table of the purity of the flow cytometric-style gating to capture target IgD- CD27- B cell subpopulations using the additional gates compared to the multi-omics label of memory switched.