FIG. 3.
Northern blot analysis of mitochondrial transcripts from various strains carrying the ori1 genome. Portions (10 μg) of total cellular RNA from various derivatives of a-1/1R/Z1 were displayed on a 2% agarose gel and transferred to a membrane prior to hybridization with the cloned ori1 sequence from plasmid pEKSori1-2. The sources of RNA were a-1/1R/Z1 (lane 1), aori1rpo41Δ (lane 2), aori1mtf1Δ (lane 3), and aori1abf2Δ (lane 4), all of which carried the mtDNA genome of a-1/1R/Z1. Two [rho0] controls were also included: αRPO41 (lane 5) and αrpo41::URA3 (lane 6). Transcription of the mitochondrial genome of a-1/1R/Z1 leads to products of small molecular weight, a finding that is in agreement with published results for this and other petite mutants (1).