FIG. 4.

Production of free 5′ DNA ends in the ori1 genome is dependent upon mitochondrial transcription. (A) Detection of 5′ ends in the mtDNA from a-1/1R/Z1, aori1rpo41Δ, and aori1mtf1Δ (the sources of DNA are indicated above the autoradiograms). (B) The same experiment carried out with mtDNA from diploid strains carrying the RPO41/rpo41::URA3 and rpo41::URA3/rpo41::URA3 alleles, respectively. All experimental details are identical to those described for Fig. 1B. Abbreviations: H, HpaII; E, EcoRV; D, Dral.