Table 3.

Gene-specific primers sequences for quantitative real-time PCR.

Gene	Primer sequence (5′to3′)	GenBank Accession Number
β-actin	F: ACCGGACTGTTACCAACACC R: CCTGAGTCAAGCGCCAAAAG	NM_205518.1
IL-1β	F: TGCCTGCAGAAGAAGCCTCG R: CTCCGCAGCAGTTTGGTCAT	NM_204524.1
IL-8	F: AGATGTGAAGCTGACGCCAAG R: CCAAGCACACCTCTCTTCCAT	DQ393272.2
IL-10	F: CTGTCACCGCTTCTTCACCT R: GAACTCCCCCATGGCTTTGT	NM_012854.2
TNF-α	F: GAACCCTCCGCAGTACTCAG R: AACTCATCTGAACTGGGCGG	HQ739087.1
ZO-1	F: AGCCCCTTGGTAATGTGTGG R: TTGGGCGTGACGTATAGCTG	XM_015278981.2
OCLN	F: ATGCACCCACTGAGTGTTGG GAGGTGTGGGCCTTACACAG	NM_205128.1
CLDN1	F: CTTCAGCACCTTCCTTTCC R: CGCTACATCTTCTGTTGGC	NM_001013611.2
NF-κB	F: AAGATCTGGTGGTGTGCCTG R: AGTGGAACCTTTCGCGGATT	NM_001012887.2
IκBα	F: CAGCACTACACTTGGCCGTA R: GGAGTAGCCCTGGTAGGTCA	NM_001001472.2
CAT	F: GGTTCGGTGGGGTTGTCTTT R: CACCAGTGGTCAAGGCATCT	NM 001031215.1
SOD1	F: GGCAATGTGACTGCAAAGGG R: CCCCTCTACCCAGGTCATCA	NM_205064.1
Keap1	F: CATCGGCATCGCCAACTT R: TGAAGAACTCCTCCTGCTTGGA	XM_025145847.1
NQO1	F: CGCACCCTGAGAAAACCTCT R: AAGCACTCGGGGTTCTTGAG	NM_001277619.1
HO-1	F: AGCTTCGCACAAGGAGTGTT R: GGAGAGGTGGTCAGCATGTC	NM_205344.1
Nrf2	F: GGGCAAGGCGTGAAGTTTTT R: GGCTTTCTCCCGCTCTTTCT	NM_205117.1
iNOS	F: ACTGAAGGTGGCTATTGGGC R: TAGCAGTTGTTGGGTGGGTG	D85422.1

β-actin, Beta actin; IL, Interleukin; TNF-α, Tumor necrosis factor; ZO-1, Zonula occludens-1; OCLN, Occludin; CLDN-1, Claudin-1; NF-κB, Nuclear factor kappa beta; IκBα, NF-kappa-B inhibitor alpha; CAT,catalase; SOD1, superoxide dismutase 1; KEAP1, kelch-like ECH-associated protein 1; NQO1, NAD(P)H quinone dehydrogenase 1; HO-1, heme oxygenase 1; Nrf2, nuclear factor erythroid 2-related factor 2; iNOS, Inducible nitric oxide synthase.