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Identification of granulosa cells and the FSH treatment. (A) Immunofluorescence analysis was performed to identify chicken granulosa cells. FSHR: green, granulosa cells marker; DAPI: blue, nucleus; Merge: identification of granulosa cells. (B) Expression of CYP21A1 mRNA is affected by FSH treatment in granulosa cells of chicken pre-hierarchal and hierarchal follicles. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01.
