Table 1.
Primers used for qRT-PCR.
| Genes | Accession number (GenBank) | Product size (bp) | Strand | Sequence (5′-3′) | Annealing temperature(°C) |
|---|---|---|---|---|---|
| β-actin | NM_205518 | 150 | F | TGCTGTGTTCCCATCTATCG | 60 |
| R | TTGGTGACAATACCGTGTTCA | ||||
| CYP21A1 | NM_001099358.2 | 145 | F | ATGAGTTCCTGCCCGAGCG | 60 |
| R | CCACACCTCCAGCAGTTCCACCACA | ||||
| STAR | NM_204686.3 | 148 | F | AATCGCTGTAGGATGTGCCC | 60 |
| R | TGGTTGATGATGGTCTTTGGCAGC | ||||
| FSHR | NM_205079.2 | 158 | F | ACCAATGCCACAGAACTGAGA | 60 |
| R | TGTAGTTTGGGAAGGCTGGA | ||||
| HSD3B1 | XM_015294362.4 | 104 | F | GCCAAAGAGGAGCAAACCAGAG | 60 |
| R | TCCAGCAGTAAGCGAACGATCC | ||||
| CYP11A1 | NM_001001756.2 | 158 | F | TCCGCCACCTCAACACCAAGA | 60 |
| R | CACAAGGAGGCTGAAGAGGATGC |