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. 2024 Mar 18;11(6):nwae100. doi: 10.1093/nsr/nwae100

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© The Author(s) 2024. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — SPIOCA regulates microbiota-derived sphingolipid metabolism. (a–c) Pathway enrichment analysis of the significantly changed metabolites in colonic contents, serum and brain tissues. (d) Graphical representation of sphingolipid metabolism. (e) Immunofluorescence of the expression of S1PR2 in the mouse cochlea (n = 3). Scale bars: 20 μm. (f) Quantification of S1PR2 expression in the cochlea. OC, organ of Corti; SGN, spiral ganglion neuron. (g) Immunofluorescence of S1PR2 expression in cochlear hair cells. Hair cells were stained for myosin 7a (n = 3). Scale bars: 20 μm. (h) Quantification of S1PR2 expression in the hair cells. *P < 0.05; **P < 0.01; ****P < 0.0001.