Skip to main content
. 2024 May 3;10(18):eadn6537. doi: 10.1126/sciadv.adn6537

Fig. 5. Ftx deficiency in female mice induces anti-NA and anti-RNP-Sm autoantibody production and the development of ABC-like cells.

Fig. 5.

(A) Anti–RNP-Sm IgG, anti-DNA IgG, and anti-RNA IgG autoantibody levels in sera of 3-month-, 1-year-, and >1.5-year-old WT or Ftx−/− females measured by ELISA. Each circle represents a mouse. Mean values are shown (Mann-Whitney test, *P < 0.05, **P < 0.01, and ***P < 0.005). (B) Representative flow cytometry analysis of ABC-like cells (CD11c+CD11b+) among DN (CD21−CD23−) in spleen from 1-year-old to 1.5-year-old WT and Ftx−/− females. Percentages are shown on the graphs. Each triangle represents a mouse. Median values are indicated. (Welch’s t test, *P < 0.05). The right panel shows the correlation between the frequency of ABCs (CD11c+CD11b+) relative to anti–RNP-Sm IgG levels in each Ftx−/− female (Pearson correlation). (C) Representative flow cytometry analysis of GC cells (Fas+GL7+) among switch memory B cells (IgM−IgD−) in spleen from 1-year-old to 1.5-year-old WT and Ftx−/− females. Percentages are shown on the graphs. Each triangle represents a mouse. Median values are indicated (Welch’s t test, *P < 0.05). The right panel shows the correlation between the frequency of GC cells (Fas+GL7+) and anti–RNP-Sm IgG Ab levels in each Ftx−/− females (Pearson correlation). (D) Representative flow cytometry analysis of monocyte populations including nonclassical (CD11bhiCD43+Ly6Clo) scavenger monocytes in the blood of 1-year-old WT and Ftx−/− females. Percentages in leucocytes are shown on the graphs beneath. Each triangle represents a mouse. Median values are shown (Mann-Whitney test, **P < 0.01).