Fig. 6.
MNK2 S74A HEK293 cells exhibit increased cell size, and enhanced invasive and transformation capacities. a G0/G1 size of CRISPR MNK2a WT and S74A cells was determined by flow cytometric analysis. b Quantification of a. c Cell adhesion assays for CRISPR MNK2a WT and S74A cells. d Quantification of data in c. e Cell migration assays for CRISPR MNK2a WT and S74A cells. f Quantification of data in e. g Cell invasion assays for CRISPR MNK2a WT and S74A cells. h Quantification of g. i Soft agar assays were performed in six-well plates and cultures maintained for 3 weeks before staining with 0.5% crystal violet. The numbers of colonies in each well larger than 100 µM were counted. j Quantification of i. For panels d, f, h and j, results are presented as means ± SD, n = 3. **0.001 ≤ P < 0.01; ***P < 0.001 (one-way ANOVA); @0.01 ≤ P < 0.05 and $P < 0.001 (two-way ANOVA)