PAS1 cDNA sequence and predicted amino acid sequence. The sequences of both cDNA-A and cDNA-D are shown. The 5′ ends, the start codons, and the 3′ ends of the two classes of cDNAs are indicated by bold face, italic, uppercase letters (cDNA-D) and by underlined letters (cDNA-A). The unspliced intron in cDNA-A (70 nucleotides) is indicated by lowercase letters. The two putative cdc2-type protein kinase C phosphorylation sites are boxed, while the Tyr phosphorylation site is indicated by a boldface broken line. The putative NLSs are underlined. The point mutations in pas1-2 at nucleotides 417 and 1317 are indicated in boldface, and altered residues are circled.