TABLE 1.

Functional complementation of the pas1-2 mutant as indicated by a segregation analysis for the pas1 mutant^a

Transformant	No. of plants with indicated phenotypeb				χ2c
	wt Kr	wt Ks	pas1 Ks	Total
pKY/D1	219	56	18	293	0.005
pKY/D3	315	74	23	412	0.312
pKY/D4	435	91	31	557	0.442
pKY/D13	211	56	18	285	0.002

^aThe segregation analysis was performed for the F2 generation of transgenic plants obtained by transformation of pas1-2 heterozygous plants with PAS1 cDNA-D. The segregation of four independent transformants heterozygous for the mutation is presented, showing a segregation ratio of 1 kanamycin-sensitive pas1 mutant to 15 heterozygous, wild-type plants. 

^bwt, wild type; Kr, kanamycin resistant; Ks, kanamycin sensitive. 

^cCalculated values were based on an expected ratio of 1 mutant plant for every 15 wild-type plants. P < 0.05.