FIG. 8.

v-Crk promotes the formation of stress fibers and focal adhesions that contain vinculin and paxillin. Native PC12 cells (A and B) or V15F cells (C to H) were cultured in 15% serum on poly(l-lysine)-coated glass coverslips. The cells were immunostained with antipaxillin (A and C) or antivinculin (E) antibodies and counterstained with rhodamine-conjugated phalloidin (B, D, and F) to detect actin microfilaments. Solid arrows demarcate areas of well-defined peripheral focal adhesions that end in stress fibers (compare the arrows in panels C and D and those in panels E and F). Panels G and H show merged composites of vinculin and actin (G) and paxillin and actin (H). Focal adhesions that stained yellow (arrows) indicate regions of colocalization. Magnification, ×91. Scale bar, 3.3 μm in panels A and B; 10 μm in panels C through H.