Table 1.
Pathway analysis of differentially methylated regions and cytosines in airway epithelium and vagal ganglia of second-generation F2 mice
| Airway Epithelium Group Comparison | |||
|---|---|---|---|
| Panther Pathway | No. of Genes | Fold Enrichment | FDR |
| F0 HDM•F2Base vs. F0 Veh•F2Base | |||
| None | |||
| F0 Veh•F2Sens vs. F0 Veh•F2Base | |||
| Beta1/2 adrenergic receptor signaling |
Cacna1c, Cacna1d, Cacnb4, Gngt2
Adcy7, Cacnb1, Cacnb3, Gnb1, Ryr1 |
12.22 | 0.018 |
| Oxytocin receptor signaling |
Cacna1d, Cacnb1, Cacnb4, Gngt2, Plcb4
Cacnb3, Gnb1, Plcd3, Prkce |
11.65 | 0.017 |
| 5HT2 type receptor signaling |
Cacna1c, Cacna1d, Cacnb4, Gngt2, Plcb4
Cacnb1, Cacnb3, Gnb1, Plcd3, Prkce |
10.11 | 0.016 |
| F0 HDM•F2 Sens vs. F0 HDM•F2 Base | |||
| Endothelin signaling |
Adcy1, Adcy4, Adcy5, Ece1, Edn2, Furin, Gna14, Itpr1, Itpr2, Map2k1, Pik3c3, Pik3r1, Pik3r5, Plcb4, Prkcd, Sec11a
Adcy7, Akt1, Gnaq, Pik3cd, Plcb3, Prkacb, Prkar1b, Prkce |
5.88 | <0.001 |
| Gonadotropin-releasing hormone receptor signaling |
Adcy1, Bmp7, Camk2b, Crtc1, Gnb1, Insr, Itpr1, Itpr2, Ksr1, Map2k1, Nfatc2, Pbx1, Pik3r1, Pitx1, Prkcd, Ppp3ca, Pxn, Smad3, Sp1
Akt1, Cacna1c, Cacna1d, Gata4, Gnaq, Hras, Hspa1a, Inhbb, Lhx2, Map2k6, Map3k8, Nfatc4, Otx1, Pcp4, Ppp3ca, Prkag2, Prkar1b, Prkce, Smad2, Tgfb1 |
2.43 | 0.029 |
| Platelet-derived growth factor signaling | Arhgap26, Arhgap27, Elf4, Ephb2, Erg, Ets1, Gm42906, Itpr1, Itpr2, Map2k1, Pik3c3, Pik3r1, Pik3r5, Rps6ka1, Vav2 | 3.16 | 0.008 |
| Wnt signaling |
Arid1b, Axin2, Bcl9, Cdh13, Csnk1g3, Dvl1, Fzd5, Fzd10, Gna14, Gnb1, Itpr1, Itpr2, Lrp5, Nfatc2, Nkd1, Pcdh1, Pcdhgb2, Plcb4, Ppp2r5c, Ppp3ca, Prkcd, Sag, Smarcd1, Tle3, Wnt3a
Acta1, Acte1, Ankrd6, Cdh2, Cdh3, Cdh23, Ctbp1, Fat2, Fzd1, Fzd7, Gm37388, Gnaq, Gng7, Kremen1, Lef1, Myh6, Nfatc4, Pcdhga10, Plcb3, Ppard, Prkce, Tle4, Wnt4, Wnt5a, Wnt5b, Wnt10b |
2.52 | 0.004 |
| Vagal Ganglia Group Comparison | |||
|---|---|---|---|
| Panther Pathway | Gene Name | Fold Enrichment | FDR |
| F0 HDM•F2Base vs. F0 Veh•F2Base | |||
| None | |||
| F0 Veh•F2Sens vs. F0 Veh•F2Base | |||
| Metabotropic glutamate receptor group III | Grik3, Grik4, Grm6, Prkar1b, Prkar2b | 7.58 | 0.038 |
| Platelet derived growth factor signaling |
Arhgap26, Arhgap27, Fli1, Gab1, Grap2, Prr5
Arhgap15, Elf4, Ephb2, Gm49337, Itpr1, Itpr3, Map2k2, Mtor, Rasal1, Rps6ka3, Spdef, Stat3, Vav1 |
5.05 | 0.053 |
| F0 HDM•F2Sens vs. F0 HDM•F2Base | |||
| None | |||
Pathways and genes enriched for both differentially methylated regions (DMRs) and differentially methylated cytosines (DMCs) are denoted in bold font. All pathways identified in DMR analysis were similarly identified in DMC analysis. Additional genes identified on DMC analysis alone are listed in normal font.