Figure 6.

Reexposure of offspring to CS at 60 wk induced changes in lung DNAm which were similar to those observed at 16 wk. A: Cyp1a1 DNAm in offspring lungs at 63 wk. Offspring with full early-life CS exposure and reexposed to CS at 63 wk (N = 3) had higher lung Cyp1a1 DNAm (mean = 17.00, SD = 0.65) compared with early-life control offspring exposed to CS at 63 wk only (N = 5, mean = 19.20, SD = 1.37). B: Cyp1a1 expression in offspring lungs at 63 wk (N = 27). Differences in DNAm and expression were calculated using a one-way ANOVA, followed by two-group comparisons. CS, cigarette smoke.