Figure 5.

PASMC isolated from patients with IPAH demonstrate a loss of hypoxia-induced p27 expression. A: p27 expression in PASMC from control and IPAH patients (n = 3 replicate experiments with 10 fields analyzed for each). p27 (green), nuclei (blue); ×200 magnification, calibration bar 50 µm. B: p27 protein expression and quantification in human PASMC exposed to normoxic (21% O₂) or hypoxic (5% O₂, 2 days) conditions (n = 4 replicate experiments). Graphs represent the means ± SD, **P < 0.01, ****P < 0.0001, as indicated. P values were measured by two-way ANOVA. IPAH, idiopathic pulmonary arterial hypertension; PASMC, pulmonary artery smooth muscle cell.