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. Author manuscript; available in PMC: 2024 May 3.
Published in final edited form as: Cell Host Microbe. 2023 Sep 25;31(10):1714–1731.e9. doi: 10.1016/j.chom.2023.08.020

Figure 3. LN resident and migratory DCs are susceptible to HIV infection in vitro.

Figure 3.

(A and B) Representative flow cytometry profiles of LN resident and migratory DCs isolated from a representative HIV-uninfected individual showing their relative susceptibility to (A) CCR5-tropic HIV-derived vector encoding for EGFP in the presence or absence of SAMHD1-modulating factor, i.e., Vpx at day 4 post-exposure or (B) to CXCR4-tropic HIV-derived vector encoding for EGFP.

(C) Percentages of EGFP+ LN DCs at day 4 post-exposure to either CCR5-tropic (N = 12) or CXCR4-tropic (N = 7) HIV-derived vectors. Activated LN CD4 T cells were added as controls (N = 5).

(D) Percentage of EGFP+ LN DCs at day 4 post-exposure to CCR5-tropic HIV-derived vector encoding for EGFP in the presence or absence of Vpx (N = 9).

(E) Levels of HIV RNA (copies/mL) in culture supernatants of unstimulated LN CD4 T cells, activated LN CD4 T cells, LN migratory DCs, and LN resident DCs of HIV-uninfected individuals exposed to HIV Ba-L (N = 3).

(F–I) Levels of HIV RNA (copies/mL) in culture supernatants of unstimulated LN CD4 T cells (F), activated LN CD4 T cells (G), LN migratory DCs (H), and LN resident DCs (I) of HIV-uninfected individuals exposed to HIV Ba-L in the presence or absence of emtricitabine (N = 3).

Red bars correspond to mean ± SEM (C–I). Red stars indicate statistical significance (*p < 0.05) of intra-group comparisons (C and D) or of conditions cultured without emtricitabine compared with conditions with emtricitabine (G and I). Black, gray, and violet stars indicate statistical significance (*p < 0.05) of activated CD4 T cells, LN resident, and migratory DCs, respectively, compared with the levels of unstimulated CD4 T cells at the corresponding time points (E). Wells with detectable HIV-1 RNA (≥2,000 HIV-1 RNA copies/mL) were referred to as HIV-1 RNA-positive wells (E–I). Gray dashed lines (E–I) represent the limit of detection. Statistical significance (p values) was obtained using one-way ANOVA (Kruskal-Wallis test) followed by Wilcoxon matched-pairs two-tailed signed rank test (C–E) or following a one-tailed ratio paired t test (F–I). “Res DC” corresponds to LN resident DCs and “Mig DC” corresponds to LN migratory DCs.