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. Author manuscript; available in PMC: 2024 May 3.
Published in final edited form as: Cell Host Microbe. 2023 Sep 25;31(10):1714–1731.e9. doi: 10.1016/j.chom.2023.08.020

Figure 4. LN resident and migratory DCs are enriched with genome-intact HIV DNA.

Figure 4.

(A) Maximum-likelihood phylogenetic trees of all HIV-1 sequences obtained from LN resident and migratory DCs of the 3 chronic viremic HIV-infected individuals (#1024, #1025, and #1019; 8–9 kb amplicons) as assessed by FLIP-seq and MIP-seq assays. Sequences from LN PD-1+/Tfh and LN PD-1−/non-Tfh cells are also depicted. Chromosomal integration site coordinates for the respective sequences are indicated.

(B) Pie charts reflecting the relative proportion of intact and defective HIV-1 sequences.

(C and D) Frequencies of cells harboring either intact (C) or defective (D) HIV-1 sequences (cells/million) as assessed by both FLIP-seq and MIP-seq assays (2 assessments per individual). HIV-infected individuals are color coded (C and D). LN cell populations are color coded (C and D). Histograms represent minimum to maximum of the range and the line corresponds to the median (C and D). Red bars correspond to 95% confidence interval. “Res DC” corresponds to LN resident DCs and “Mig DC” corresponds to LN migratory DCs.