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REST1-VP16 and REST-VP16 stimulate basal promoters through RE1/NRSE sequence. HeLa cells were transfected with the reporter plasmid pT.luc, pRE.T.luc or pREm.T.luc in the presence and absence of the expression vector alone, or the expression vector encoding REST, REST1-VP16, REST-VP16 or Gal4-VP16 and analyzed as described in Materials and Methods. Promoter activity is expressed as relative luciferase units (RLU).
