A spike virosome vaccine induces pan-sarbecovirus antibody responses in mice

. 2024 Apr 10;27(5):109719. doi: 10.1016/j.isci.2024.109719

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies

COVA2-02	(Brouwer et al., 2020)³⁰	N/A
COVA1-16	(Brouwer et al., 2020)³⁰	N/A
S309	(Pinto et al., 2020)³²	N/A
J08	(Andreano et al., 2021)³¹	N/A
Goat anti-mouse IgG-PE	Southern Biotech	Cat# 1030-09, 1030-09S; RRID:AB_2794297, RRID:AB_2794298

Chemicals, peptides, and recombinant proteins

PBS	Thermo Fisher	Cat# 10010023
PEI MAX	Polysciences	Cat# 24765-1
3,3′,5,5′-tetranethylbenzidine	Sigma-Aldrich	Cat# T4444
Poly-L-Lysine Hydrobromide	Sigma-Aldrich	Cat# P1399
Penicillin	Sigma-Aldrich	Cat# P3032-10MI
Streptomycin	VWR	Cat# 382-EU-100G
Gibco™ MEM Non-Essential Amino Acids Solution (100X)	Thermo Fisher	Cat #11140050
Gibco™ HEPES 1M	Thermo Fisher	Cat #15630056
Blasticidin solution	Invivogen	Cat #ant-bl-05
SARS-CoV-2 S Foldon his	This Study	N/A
SARS-CoV-2 Gamma S Foldon his	This Study	N/A
SARS-CoV-2 Delta S Foldon his	This Study	N/A
SARS-CoV-2 Gamma S Foldon his	This Study	N/A
SARS-CoV S Foldon his	This Study	N/A
SHC014 S Foldon his	This Study	N/A
WIV1 S Foldon his	This Study	N/A
Pang17 S Foldon his	This Study	N/A
RShST200 S Foldon his	This Study	N/A
Rf1 S Foldon his	This Study	N/A
Rs4081 S Foldon his	This Study	N/A
Btky72 S Foldon his	ARCO Biosystems	SPN-S52Hu
Khosta-2 S Foldon his	This Study	N/A
PRD-0038 S Foldon his	This Study	N/A
HCoV-229E S Foldon his	This Study	N/A
HCoV-NL63 S Foldon his	This Study	N/A
HCoV-OC43 S Foldon his	This Study	N/A
HCoV-HKU1 S Foldon his	This Study	N/A
MERS-CoV S Foldon his	This Study	N/A
Influenza virus HA	The Native Antigen Company	REC32023-100
HIV-1 BG505 Env	(Sanders et al., 2023)⁵⁷	N/A
Influenza virus virosomes	Mymetics B.V.	N/A
3M-052	3M	N/A
QS-221	MedChem Express	Cat# HY-101092
Polyinosinic-polycytidylic acid	Invivogen	vac-pic

Critical commercial assays

Nano-Glo Luciferase Assay System	Promega	Cat# N1130
4-16% Bis-Tris NuPAGE gels	Invitrogen	BN1002BOX
Nucleobond Xtra Maxi kit	Macherey-Nagel	Cat# 740414.50

Experimental models: Cell lines

FreeStyle 293F cells	Thermo Fisher	Cat# R79007
HEK 293T/ACE2 cells	(Schmidt et al., 2022)⁶¹	N/A
HEK 293T cells	ATCC	Cat# CRL-11268
Huh7 cells	A gift from dr. François-Loïc Cosset	N/A

Experimental models: Organisms/strains

BALB/cAnNCrl mice	Charles River Laboratories	N/A

Recombinant DNA

pHIV-1_NL43ΔENV-NanoLuc plasmid	(Schmidt et al., 2022)⁶¹	N/A
SARS-CoV-2-S_Δ19 plasmid	(Schmidt et al., 2022)⁶¹	N/A
SARS-CoV-S_Δ19 plasmid	(Brinkkemper et al., 2022)⁴⁰	N/A
SHC014S_Δ19 plasmid	This study	N/A
WIV1-S_Δ19 plasmid	This study	N/A
Pangolin-CoV-GD/19-S_Δ19 plasmid	This study	N/A
RaTG13-S_Δ19 plasmid	This study	N/A
229E-CoV-S_Δ19 plasmid	This study	N/A
MERS-CoV-S_Δ19 plasmid	This study	N/A
Neo-CoV-S_Δ19 plasmid	This study	N/A
hCoV-NL63 plasmid	Addgene	#172666
SARS-CoV-2-S-Foldon-his plasmid	(Brouwer et al., 2020)³⁰	N/A
SARS-CoV-2-Gamma-S-Foldon-his plasmid	This study	N/A
SARS-CoV-2-Delta-S-Foldon-his plasmid	This study	N/A
SARS-CoV-S-Foldon-his plasmid	This study	N/A
SHC014-S-Foldon-his plasmid	This study	N/A
WIV1-S-Foldon-his plasmid	This study	N/A
Pang17-S-Foldon-his plasmid	This study	N/A
RshSTT200-S-Foldon-his plasmid	This study	N/A
Rf1-S-Foldon-his plasmid	This study	N/A
Rs4081-S-Foldon-his plasmid	This study	N/A
Khosta-2-S-Foldon-his plasmid	This study	N/A
PRD-0038-S-Foldon-his plasmid	This study	N/A
229E-S-Foldon-his plasmid	(Grobben et al., 2022)³⁵	N/A
NL63-S-Foldon-his plasmid	(Grobben et al., 2022)³⁵	N/A
OC43-S-Foldon-his plasmid	(Grobben et al., 2022)³⁵	N/A
HKU1-S-Foldon-his plasmid	(Grobben et al., 2022)³⁵	N/A
MERS-CoV-S-Foldon-his plasmid	(Grobben et al., 2022)³⁵	N/A
BG505-Env plasmid	(Sanders et al., 2023)⁵⁷	N/A

Software and algorithms

GraphPad Prism v8	GraphPad	N/A
UCSF ChimeraX	(Goddard et al., 2018)⁶⁴	N/A
Adobe Illustrator	Adobe	N/A

Other

Superose 6 increase 10/300 GL	Sigma-Aldrich	Cat# GE29-0915-96
Octet K2 system	Sartorius (FortéBio)	N/A
Octet Biosensors: Protein A	Sartorius (FortéBio)	Cat# 18-5010
Vivaspin 20, 100.000 kDa MWCO, Polyethersulfone	Sigma-Aldrich	Cat# GE28-9323-63
Fast Digest BamHI	Thermo Scientific	Cat# FD0054
Fast Digest Green buffer 10x	Thermo Scientific	Cat# B72
Fast Digest PstI	Thermo Scientific	Cat# FD0614
FreeStyle 293 Expression medium	Thermo Scientific	Cat# 12338018
DMEM	Sigma-Aldrich	Cat# D6429-500ML
Glutamax supplement	Thermo Fisher	Cat# 35050061
Steritop Filter Units	Merckmillipore	Cat# C3239
Glomax	Turner BioSystems	Model# 9101-002
Microplate 96 well half area white	Greiner bio-one	Cat# 675074
AKTA Avant150 FPLC system	Cytiva	N/A
MagPlex-C Microspheres	DiaSorin	MC100xx
MAGPIX system	DiaSorin	MAGPIX-XPON4.1-CEIVD