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. 1998 May;18(5):3069–3080. doi: 10.1128/mcb.18.5.3069

FIG. 1.

FIG. 1

p62 interacts with the aPKC in vitro. (Upper panel) Purified MBP or MBP-p62 (400 nM) was immobilized on amylose beads and incubated with 1 mg of protein extracts from HeLa cells at 22°C for 1 h. After extensive washing, the recombinant proteins were fractionated by SDS-PAGE and the associated PKC isotypes were determined with the corresponding isotype-specific antibodies by immunoblotting. Ext., 50 μg of protein extracts was run in parallel as a control. MW, molecular weight (in thousands). (Lower panel) Amounts of MBP fusion proteins in each experiment were the same. Essentially identical results were obtained in two more experiments.