FIG. 9.

Colocalization of p62 with different endosomal and lysosomal markers. HeLa cells were transfected with 5 μg of an expression vector for HA-tagged p62 and analyzed 20 h posttransfection by confocal laser scanning microscopy with monoclonal anti-HA antibody and FITC-conjugated anti-mouse IgG (green fluorescence) (A) or polyclonal anti-HA antibody (B and C) and Texas red-conjugated anti-rabbit IgG (red fluorescence) (B) or FITC-conjugated anti-rabbit IgG (green fluorescence) (C). Rab7 (A) was detected with an anti-rab 7 polyclonal antibody and Texas red-conjugated anti-rabbit IgG (red fluorescence). Lamp-1 (B) was detected with a monoclonal anti-lamp-1 antibody and FITC-conjugated anti-mouse IgG (green fluorescence). Limp II (C) was detected with a monoclonal anti-Limp II antibody and Texas red-conjugated anti-mouse IgG (red fluorescence). Bar, 5 μm. Essentially identical results were obtained in three more experiments.