Figure 5.

Cytocentrifugation and scoring of patient-derived CD34⁺ cells on day 11 of erythroid differentiation

(A) Representative cytocentrifugation images of stained treated and control cells. Size marker: 20 μm. (B) Bar chart representing differential scoring of the proportion of erythroid subpopulations. Groupwise comparison was made to untreated control using parametric mixed-effects analysis with Dunnett’s multiple comparison test. For basophilic: ∗p = 0.0283. For polychromatophilic: ∗∗p = 0.0030 and ∗p = 0.0151. For orthochromatophilic: ∗∗p = 0.0032 for SpRY6, ∗∗p = 0.0016 for SpRY20, and ∗p = 0.0171. (C) Bar chart indicating the number of cells in late-stage erythropoiesis (equal to the sum of orthochromatophilic cells and reticulocytes from B) and hemoglobin-positive (Hb⁺) cells, equivalent to late-stage erythropoietic cells plus polychromatophilic (dianisidine-staining cells). Analyses are of patient-derived cells following treatment (n = 3 for SpRY20, n = 5 for other treatments). Groupwise comparison was made to untreated controls and significance obtained using parametric mixed-effects analysis with Dunnett’s multiple comparison test. ∗∗p = 0.0028 and ∗p = 0.0137 for SpG7 and ∗p = 0.0295 for SpRY20. For (B) and (C), error bars show the standard deviation of the mean, and only the upward variation is given.