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. 2024 May 3;15:3729. doi: 10.1038/s41467-024-48031-8

Fig. 2. Synthetic BZLF1-specific TALE transcriptional activator.

Fig. 2

a Schematics of the TALE transcriptional activator targeting the BZLF1 promoter in the EBV genome. b Western blotting detected the endogenous expression of immediate early (Zta, Rta), early (BGLF4), and late (VCAp18) lytic proteins in SNU719 and C666-1 cells transiently transfected with the designed BZLF1-targeted TALE plasmid, TZ3 and other TALE plasmids, TZ1, TZ2 and TZ4. P3HR1 cells treated with NaB and TPA were included as the controls for lytic protein expression (n = 3 experimental replicates). c Using immunofluorescence (IF) staining, Zta expression was detected in SNU719 and C666-1 cells transiently transfected with TZ3 (n = 3 experimental replicates). Representative IF images are shown. Scale bar = 10 μm. d Using RNA sequencing, differentially expressed genes between TZ3-transfected and control HK1 cells were determined (n = 3 experimental replicates). Few genes showed significant changes in expression in TZ3-transfected HK1 cells. e No significant reduction of cell viability was detected in the TZ3-transfected HK1 cells (n = 3 experimental replicates). Data are presented as mean ± SD. ns not significant. f Ectopic transfection of TZ3 did not significantly alter the cell cycle of HK1 cells (n = 3 experimental replicates). Data are presented as mean ± SD. ns not significant; e, f Two-sided student’s t-test. Source data are provided as a Source Data file.