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. 2024 May 3;15:3734. doi: 10.1038/s41467-024-47219-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of the hPGCLC differentiation protocol adapted from⁴⁷. b Representative flow cytometry plots and (c) quantification of hPGCLC frequency at day 4 from three independent wildtype or REV1^-/- clones differentiated three times. (Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). d Representative images of wildtype and REV1^-/- aggregates immunostained for SOX17, TFAP2C and OCT4. e Offspring when male (left) and female (right) wildtype or Rev1^-/- mice were mated with wildtype mates of the opposite sex (n = 6 mice per genotype, 3 per sex. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). f Quantification of testis mass of 8−12-week-old wildtype and Rev1^-/- mice (n = 12, 2, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). g Quantification of ovary mass from 8−12-week-old wildtype and Rev1^-/- mice (n = 13, 2, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). h H&E-stained ovaries and testes seminiferous tubules from 8−12-week-old wildtype and Rev1^-/- mice. i Quantification of SCO tubules per section of testis of 8−12-week-old wildtype and Rev1^-/- mice (n = 10 and 4 animals, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). j Quantification of follicles per section of ovary from 8−12-week-old wildtype and Rev1^-/- mice (n = 8 and 3 animals, left to right). Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test. k Schematic for generation of Rev1^-/- embryos harboring the GOF18-GFP reporter. l GFP fluorescence images of gonads from wildtype and Rev1^-/- E12.5 embryos. m Representative flow cytometry plots and (n) quantification of PGCs by flow cytometry from wildtype and Rev1^-/- E12.5 embryos (n = 35 and 12, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test).