Fig. 3. Embryonic origin of sterility upon PCNA K164 mutation.

a Representative images of testes and ovaries from wildtype and Pcna^R/R mice.Quantification of (b) testicular or (c) ovarian mass from 8-12-week-old wildtype and Pcna^R/R mice (n = 12, 10, 13 and 8 left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). d Cumulative number of offspring when wildtype or Pcna^R/R mice were mated with wildtype mates of the opposite sex (n = 6 mice per genotype, 3 per sex. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). e H&E-stained testis seminiferous tubules and (f) quantification of SCO tubules per section of testis of 8−12-week-old wildtype and Pcna^R/R (n = 10 and 15, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). g H&E-stained ovaries and (h) quantification of follicles per section of ovary from 8−12-week-old wildtype and Pcna^R/R mice (n = 8 and 11, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). i GFP fluorescence images of gonads from wildtype and Pcna^R/R E12.5 embryos. j Representative flow cytometry plots and (k) quantification of PGCs from wildtype, Rev1^-/- and Pcna^R/R gonads at E12.5 (n = 35, 12 and 21, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). l Quantification of PGCs by flow cytometry from wildtype, Rev1^-/-, Pcna^R/R and Rev7^-/- embryos at E8.5 (n = 18, 5, 2 and 3, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). m Quantification of PGCs by flow cytometry from E8.5 to E12.5 in wildtype and mutant embryos (wildtype, n = 18, 12, 9, 3 and 17; Rev1^−/−, n = 5, 6, 5, 2 and 8; Pcna^R/R, n = 2, 4, 4, 7 and 9; Rev7^−/−, I = 3, 0, 0, 0 and 7, independent embryos, left to right. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test).