Fig. 4.

Knockdown of NADK enhances Erastin-induced ferroptosis in LUAD cells by downregulating NADPH levels. A, B Total levels of NADP⁺ and NADPH as well as the ratio of NADPH/NADP⁺ in NADK-stable knockdown and control H1299/A549 cells were measured using NADP⁺/NADPH Assay Kit with WST-8 after 24-h treatment with 5 μM Erastin. C, D Cell death of NADK-stable knockdown and control H1299/A549 cells were assessed by PI staining, after 24 h of treatment with 5 μM Erastin in combination with DMSO, Liproxstatin-1, and NADPH, respectively. E–F Intracellular MDA levels of NADK-stable knockdown and control H1299(E)/A549(F) cells were measured, after 24 h of treatment with 5 μM Erastin in combination with DMSO, NADPH, and Liproxstatin-1, respectively