Skip to main content
. 2024 Apr 16;27(5):109749. doi: 10.1016/j.isci.2024.109749

Figure 2.

Figure 2

Pod2.IGF1RKD mice have mildly increased albuminuria at 6 months

(A) PAS staining; no apparent change in histological appearance of pod2.IGF1RKD mice compared with littermate controls. Scale bar upper panel = 75 μm, lower panel = 25 μm.

(B) Transmission electron microscopy (TEM); no apparent change in ultrastructural appearance in pod2IGF1RKD mice. Scale bar upper panel = 5 μm, lower panel = 500 nm. No differences in slit diaphragm width, foot process width or glomerular basement membrane thickness were observed. Data are represented as the mean ± SEM.

(C) Immunofluorescence analysis of kidney sections shows reduced levels of nephrin in pod2.IGF1RKD relative to control mice. Quantification was performed by measuring glomerular fluorescence intensity in ≥5 glomeruli in 3 mice per group expressed as the mean ± SEM, t-test, ∗p < 0.05.

(D) uACR of pod2.IGF1RKO and littermate control mice at 24 weeks. Data are expressed as the mean ± SEM, t-test, ∗∗p < 0.005. n = 5–7.

(E) Immunofluorescence analysis of kidney sections showing reduced levels and reduced colocalization of P-p44/42 MAPK and nephrin in pod2.IGF1RKD relative to control mice. Scale bar = 50 μm. Quantification was performed by measuring glomerular fluorescence intensity expressed as the mean ± SEM in ≥5 glomeruli in 3 mice per group. t-test, ∗∗p < 0.005.

See also Figures S2–S4.