Figure 6.
Proteomic analysis of NC-IGF1RKD and P-IGF1RKD podocytes reveals the differential expression of mitochondrial respiratory electron transport chain proteins dependent on the degree of IG1R suppression
(A‒E) Graphs showing log2 fold change of significantly differentially expressed mitochondrial respiratory complex I (A), complex II (B), complex III (C), complex IV (D), and complex V (E) proteins in NC-IGF1RKD and P-IGF1RKD podocytes compared to wild-type. Data are represented as the mean ± SEM.
(F) Representative Western blot and densitometry show reduced expression of the respiratory complex I protein NDUFB8 in NC-IGF1RKD compared with wild-type podocytes but significantly increased the expression of respiratory complex V protein ATP5A in both NC-IGF1RKD and P-IGF1RKD podocytes relative to control. Data are represented as the mean ± SEM, t-test, ∗p < 0.05, ∗∗∗p < 0.001, n = 3 independent experiments.
(G) Immunohistochemistry shows reduced podocyte expression (arrowed) of NDUFB8 in pod2.IGF1RKO mice. Scale bar left panel = 100 μm, right panel = 50 μm. Quantification was performed by measuring glomerular signal intensity in 4 glomeruli in 2 mice per group and is expressed as the mean ± SEM, t-test, ∗p < 0.05.
See also Figure S9.