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. 2024 May 3;15:3747. doi: 10.1038/s41467-024-48140-4

Fig. 1. PfDOZI::GFP localizes to the cytosolic granules in asexual and sexual blood-stage parasites.

Fig. 1

a, b Representative live images of the PfDOZI::GFP parasites in asexual erythrocytic stages a and b stage II–V gametocytes. R, ring; ET, early trophozoite; LT, late trophozoite; S, schizont. Nuclei were counter-stained with DAPI. The numbers indicate the co-localization correlation coefficient between the GFP and DAPI fluorescence signals. Scale bar = 2 μm. c, d Co-localization of PfDOZI with Alba4 (c) and PABP1 (d) detected by IFA with the respective antibodies. T, trophozoite; S, schizont; Mz, merozoite; Gc, gametocyte. Nuclei were counter-stained with DAPI. The numbers indicate the co-localization correlation coefficient between the GFP and Alba4 or PABP1 fluorescence signals. DIC, differential interference contrast microscopy. Scale bar = 2 μm. All experiments in this figure were repeated three times independently with similar results.