Fig. 6. RIP-seq analysis of PfDOZI-associated mRNAs in P. falciparum schizonts and gametocytes.

a, b Venn diagrams showing the overlap of transcripts between PfDOZI-associated mRNAs and differentially regulated genes in the Δpfdozi parasite at the schizont (a) and gametocyte (b) stage. The enriched pathways in each group were identified by the MPMP analysis. Blue, downregulated transcripts; Red, upregulated transcripts. c Real-time qRT-PCR confirmation of PfDOZI association with the selected transcripts. mRNAs immunoprecipitated using GFP-trap antibodies from the PfDOZI::GFP parasites at the schizont (blue) and gametocyte (red) stages were analyzed by qRT-PCR with primers specific to the indicated genes. Error bars indicate the mean ± SD of three independent experiments. All fold-change values are represented as the relative expression in comparison to the internal reference gene PF3D7_0717700. Source data are provided as a Source Data file.