Fig. 3.

Rapamycin and monocytes rescue iPSC from apoptosis A Cells treated with 10 µM CHIR and 5 ng/mL Noggin for 48 h were fixed and stained using Mitotracker green and LAMP-2 antibody. Co-localization of Mitotracker green and LAMP-2 is shown by white asterisk. Higher magnification images of an autophagosome and Plot Profile of green and red fluorescence created using ImageJ software are shown in bottom panels. B Expression of autophagy marker p62, cleaved caspase 3 (C) and cleaved PARP-1 (D) in iPSC cells treated for 48 h with CHIR and Noggin was assessed by western blotting. The upper panels show typical western blotting picture. The lower panels show quantification for at least three independent experiments. Full-length blots are presented in Additional file 1 Fig. S2