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. 2024 May 3;15:132. doi: 10.1186/s13287-024-03739-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Monocytes promote differentiation of renal organoids. iPSC differentiation to renal organoids has been performed in the transwell co-culture with human monocytes from Day 0 (Mono D 0) or Day 7 (mono Day 7) of the differentiation protocol and in the presence of rapamycin (Rapa). Expression of renal markers (A–E) was assessed by TaqMan RT-PCR on Day 28 of the protocol. F Number of organoids per area unit was quantified on Day 28 of the protocol