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. 2024 May 4;14:10297. doi: 10.1038/s41598-024-61025-2

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© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Schematic Presentation of the final multi-epitope vaccine construct. (B) The secondary structural prediction of the vaccine. (C) The three-dimensional refined vaccine model is visualized to represent the helical, sheet, and loop regions. (D) Validation of the vaccine structure by ERRAT with a score of 95.5036. (E) ProSA validation of predicted structure with Z-score of −9.78 and (F) plots the residues scores to check the local model quality. (G) Analysis of the Ramachandran plot utilizing the PROCHECK server showed 94.6%, 3.5%, 0.4%, and 1.5% residues laying in favored, additional allowed, allowed, and disallowed regions, respectively.

(A) Schematic Presentation of the final multi-epitope vaccine construct. (B) The secondary structural prediction of the vaccine. (C) The three-dimensional refined vaccine model is visualized to represent the helical, sheet, and loop regions. (D) Validation of the vaccine structure by ERRAT with a score of 95.5036. (E) ProSA validation of predicted structure with Z-score of −9.78 and (F) plots the residues scores to check the local model quality. (G) Analysis of the Ramachandran plot utilizing the PROCHECK server showed 94.6%, 3.5%, 0.4%, and 1.5% residues laying in favored, additional allowed, allowed, and disallowed regions, respectively.