Table 1.
Composition of basal diets (as-fed basis)
| Item | Phase 1 | Phase 2 | Phase 3 |
|---|---|---|---|
| Feedstuff, % | |||
| Corn (yellow dent) | 41.44 | 46.48 | 51.12 |
| Soybean meal (48% CP) | 17.00 | 15.50 | 14.00 |
| Corn distiller’s dried grains with solubles | 5.00 | 15.00 | 30.00 |
| Whey permeate | 20.00 | 12.00 | 0.00 |
| Processed soybean meala | 6.00 | 3.00 | 0.00 |
| Blood plasma | 6.00 | 3.00 | 0.00 |
| Poultry fat | 0.97 | 1.22 | 0.62 |
| L-Lys HCl | 0.44 | 0.60 | 0.77 |
| L-Met | 0.19 | 0.17 | 0.13 |
| L-Thr | 0.13 | 0.16 | 0.19 |
| L-Trp | 0.00 | 0.02 | 0.05 |
| L-Val | 0.03 | 0.05 | 0.04 |
| L-Ile | 0.01 | 0.02 | 0.01 |
| Dicalcium phosphate | 0.10 | 0.10 | 0.10 |
| Limestone | 1.30 | 1.29 | 1.19 |
| Vitamin premixb | 0.03 | 0.03 | 0.03 |
| Mineral premixc | 0.15 | 0.15 | 0.15 |
| Sodium chloride | 0.22 | 0.22 | 0.22 |
| Enzyme mixtured (corn and feed enzyme) | 0.50 | 0.50 | 0.50 |
| Phytase mixturee (corn and phytase) | 0.50 | 0.50 | 0.50 |
| Titanium dioxide | 0.00 | 0.00 | 0.40 |
| Calculated composition | |||
| Dry matter, % | 91.53 | 90.62 | 89.29 |
| Metabolizable energy, kcal/kg | 3,400 | 3,400 | 3,350 |
| Crude protein, % | 22.07 | 20.53 | 20.21 |
| SIDf Lys, % | 1.50 | 1.35 | 1.23 |
| SID Met + Cys, % | 0.82 | 0.74 | 0.68 |
| SID Thr, % | 0.88 | 0.79 | 0.73 |
| SID Trp, % | 0.26 | 0.22 | 0.20 |
| SID Val, % | 0.95 | 0.86 | 0.78 |
| Total Ca, % | 0.68 | 0.63 | 0.53 |
| STTDg P, % | 0.36 | 0.30 | 0.23 |
| Total P, % | 0.54 | 0.48 | 0.43 |
| Analyzed composition | |||
| Dry matter, % | 89.54 | 88.74 | 87.83 |
| Gross energy, kcal/kg | - | - | 4,012 |
| Crude protein, % | 21.45 | 20.12 | 19.35 |
| Neutral detergent fiber, % | 6.23 | 9.91 | 12.43 |
| Acid detergent fiber, % | 3.13 | 3.63 | 5.80 |
| Ether extract, % | 3.26 | 4.04 | 4.07 |
aProcessed soybean meal was a hydrolyzed soy protein product (HP 300) from Hamlet Protein (Findlay, OH, USA)
bThe trace mineral premix provided per kilogram of complete diet: 33 mg of Mn as manganese oxide, 110 mg of Fe as ferrous sulfate, 110 mg of Zn as zinc sulfate, 16.5 mg of Cu as copper sulfate, 0.30 mg of I as ethylenediamine dihydroiodide, and 0.30 mg of Se as sodium selenite
cThe vitamin premix provided per kilogram of complete diet: 6,614 IU of vitamin A as vitamin A acetate, 992 IU of vitamin D3, 19.8 IU of vitamin E, 2.64 mg of vitamin K as menadione sodium bisulfate, 0.03 mg of vitamin B12, 4.63 mg of riboflavin, 18.52 mg of D-pantothenic acid as calcium pantothenate, 24.96 mg of niacin, and 0.07 mg of biotin
dFive dietary treatments consisted of 5 levels of NSP degrading enzyme with xylanase and β-glucanase combination (XG: 0, 280/125, 560/250, 840/375, and 1,120/500 (TXU/TGU)/kg) supplemented to basal diets; XG were supplemented at the expense of corn in the enzyme mixture; the analyzed activities of xylanase and β-glucanase in feeds were 65/44, 360/193, 637/288, 775/424, and 1,114/466 (TXU/TGU)/kg in phase 1, 121/194, 421/267, 682/445, 936/458, and 1,226/517 (TXU/TGU)/kg in phase 2, and 89/153, 424/272, 561/428, 987/562, and 1,340/708 (TXU/TGU)/kg in phase 3, respectively
ePhytase supplementation level was 750 FTU/kg in basal diets
fSID Standardized ileal digestible
gSTTD Standardized total tract digestible