Skip to main content
. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: J Genet Genomics. 2023 May 13;50(7):473–485. doi: 10.1016/j.jgg.2023.05.001

Fig. 1.

Fig. 1.

Nitric oxide stabilizes IAA17-GUS protein. A: Histochemical staining of the GUS activity in 7-day-old seedlings carrying an HS::IAA17NT-GUS reporter. After heat shock at 37°C for 2 h, the seedlings were transferred to media in the absence (mock) or the presence of 10 μM IAA, 50 μM MG132, 200 μM SNP, and 200 μM GSNO and incubated at 22°C for the indicated time prior to GUS staining. B and C: Immunoblotting analysis and quantification of IAA17NT-GUS levels in seedlings shown in (A). Anti-HSP82 is used as a loading control. D: Analysis of the GUS activity in 7-day-old HS::IAA17NT-GUS seedlings treated with the indicated chemicals and concentrations. HS/Mock and other columns, HS samples treated with DMSO (HS/Mock) and various chemicals at 22°C for 2 h. Data are mean ± SD of three independent experiments. Different letters indicate individual groups for multiple comparisons with significant differences (one-way ANOVA, Duncan, P < 0.05). HS, heat shock. Scale bar, 1 cm (A).