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. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: J Genet Genomics. 2023 May 13;50(7):473–485. doi: 10.1016/j.jgg.2023.05.001

Fig. 2.

Fig. 2.

Nitric oxide promotes IAA17 protein accumulation. A: GUS staining of 7-day-old seedlings carrying an HS::IAA17NT-GUS reporter in Col-0 (WT) or NO-overproducing mutants (gsnor1–3, gsnor1 rog1–1, and nox1) treated with heat shock at 37°C for 2 h. B: Immunoblotting analysis (top) and quantification (bottom) of IAA17 protein levels in NO-overproducing mutants. IAA17 gain-of-function mutants of axr3–1 and axr3–3, and 35S::IAA17-FLAG transgenics are used as controls. C and D: Immunoblotting analysis (top) and quantification (bottom) of IAA17 protein levels treated with the nitric oxide donors SNP and GSNO. Treatment with MG132 is used as a control. Data are mean ± SD of three independent experiments. The statistical significance is determined by a two-sided Student’s t-test (Paired two sample for means). **, P < 0.01 when compared to the mock.