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. Author manuscript; available in PMC: 2025 May 1.
Published in final edited form as: J Allergy Clin Immunol. 2024 Feb 22;153(5):1381–1391.e6. doi: 10.1016/j.jaci.2024.01.026

Figure 2. Papillae and dynamic changes of MC-AI–identified papillary MC density as a function of disease state.

Figure 2.

A. Immunofluorescence of E-cadherin (left, red) representing esophageal epithelium, low-level autofluorescence at 651-nm wavelength (middle, white), and their overlay (right). Arrowhead: papillae. B. Immunofluorescence of esophageal epithelium with nuclear (blue) and mast cell (MC) tryptase (yellow) staining (left), low-level autofluorescence at 651 nm (middle, white), and manual tracing of papillae overlaid with MC tryptase (yellow) (right). A-B images are crops of digitally stitched images of composite overlapping 40X images of one representative biopsy section each. C. MC density in control papillae (n = 19 biopsy sections), epithelium (n = 21), and lamina propria (n = 6) (left); EoE remission (Remission) papillae (n = 18), epithelium (n = 20), and lamina propria (n = 14) (middle); and active EoE (Active) papillae (n = 35), epithelium (n = 36), and lamina propria (n = 20) (right). Markers represent individual biopsy sections, and bars represent the mean. D. Correlation of log-transformed values between papillary and epithelial MC density across all disease states; markers represent individual biopsy sections. Statistical difference between groups; *p < 0.0332, **p < 0.0021; ***p < 0.0002, ****p < 0.0001, ns = not significant.