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. Author manuscript; available in PMC: 2025 May 1.
Published in final edited form as: J Allergy Clin Immunol. 2024 Feb 22;153(5):1381–1391.e6. doi: 10.1016/j.jaci.2024.01.026

Figure 4. Degranulated MC-AI.

Figure 4.

A. Crop of digitally stitched whole biopsy immunofluorescence of human esophageal tissue, multiple 40X high-powered fields (left). Nuclear (blue) and tryptase (yellow) staining for mast cells (MCs). Degranulated MC-AI–identified degranulated MCs (cyan) (right). B. Correlation between manual and Degranulated MC-AI–identified degranulated MCs (simple linear regression, n = 13 biopsy sections). C. Degranulated MC-AI–identified degranulated MC density in active EoE (Active, n = 6 biopsy sections) and control (n = 7). D. MC-AI–identified MCs were used to identify intracellular tryptase (yellow, left); tryptase was identified by fluorescence intensity thresholding of anti-tryptase (right), and intracellular tryptase (left) was subtracted to identify extracellular tryptase (white; arrowhead; middle); merged areas of intracellular and extracellular tryptase (right). E. Area of extracellular tryptase per biopsy section as identified using method in D in active EoE (n = 9 biopsy sections) and controls (n = 8). C and E. Markers represent individual patient biopsy sections; bars represent the median. F. Tryptase mean fluorescence intensity (MFI) of individual MCs in esophageal epithelium in active EoE (n = 2,858 MCs), EoE remission (Remission, n= 445), and control (n= 119) patients (left); in papillae in active EoE (n = 95), EoE remission (n = 69), and control (n = 61) patients; and in lamina propria (LP) in active EoE (n = 202), EoE remission (n = 325), and control (n = 174) patients. Box represents 25–75% of values, lines represent 1–100%. Statistical differences between groups *p < 0.0332, **p < 0.0021, ***p < 0.0002, ****p < 0.0001, ns = not significant.