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. Author manuscript; available in PMC: 2025 May 1.
Published in final edited form as: J Allergy Clin Immunol. 2024 Feb 22;153(5):1381–1391.e6. doi: 10.1016/j.jaci.2024.01.026

Figure 6. MC and eosinophil epithelial distribution.

Figure 6.

A. Digitally stitched whole biopsy immunofluorescence of human esophageal tissue in control (left) and active eosinophilic esophagitis (EoE) (Active, right) patient, multiple 40X high-powered fields. Nuclear (blue) and MC-AI–identified mast cell (MC, yellow) staining. White dashed line: basement membrane. Arrowheads: MCs. B. Distance of individual MCs as identified by MC-AI from the basement membrane in control (n = 20 MCs), EoE remission (Remission, n = 289), and active EoE (n = 514) patients. C. Immunofluorescence of human esophageal tissue in active EoE. Arrow: Eos-AI–identified eosinophils (eos). Arrowhead: MC-AI–identified MCs. D. Correlation between manual and Segment.ai counting of eosinophils (n = 39 biopsy sections) by simple linear regression. E. Distance of eosinophils (n = 438 cells) and MCs (n = 501 cells) from the basement membrane as identified by Eos-AI and MC-AI, respectively. Cell counts were derived from n = 7 patient samples. Boxes represent 25–75% of values; lines represent 1–100%. Statistical difference between the groups *p < 0.0332, **p < 0.0021; ***p < 0.0002, ****p < 0.0001, ns = not significant.