Figure 4.

Methylation state of the transgene array following two generations of imprinting. DNA was extracted from circulating erythrocytes and digested as described in Figure 3. Expressing and non-expressing individuals were collected from litters represented in Figure 1 (D, offspring with a non-expressing mother and grandmother; E, offspring with a non-expressing father and a non-expressing grandmother). A banding pattern, suggestive of extensive methylation, was obtained following female transmission (as seen in Fig. 3) and with the non-expressing individual resulting from inheritance through a non-expressing female then a non-expressing male.